Speaker
Description
TMPyP4 is well-known meso-substituted porphyrin with high biological activity and unique spectroscopic and photophysical properties. This highly symmetric (D2h) fluorescent compound is widely used as a photosensitizer in anticancer PDT, anti-viral and antimicrobial agent, an efficient probe for the nucleic acids structure and dynamics, a carrier of antisense oligonucleotides for their delivery, stabilizer of G-quadruplexes etc. The binding of TMPyP4 and its tricationic derivative to synthetic nucleic acids of different structure and base composition have been comprehensively studied by many authors and us [1–5] in a wide range of phosphate/dye ratio (P/D) using various spectroscopic methods. It was shown that the way of the porphyrin binding depends on NA base composition and spatial structure, and on P/D. An aggregation of both porphyrins on the biopolymers surface at a near-stoichiometric in charge P/D ratio has been revealed for all systems studied. The work is focused on comparison of our data with that of other authors on the spectroscopic features of the porphyrin binding to synthetic nucleic acids, their analysis and generalization.
References
[1] O. Ryazanova, I. Voloshin et al, J. Fluoresc. doi:10.1007/s10895-024-04000-4 (2024).
[2] O. Ryazanova, I. Voloshin et al, Mol. Cryst. Liq. Cryst. 698(1), 26 (2020).
[3] O.Ryazanova, V. Zozulya et al, Methods Appl. Fluoresc. 4(3), 034005 (2016).
[4] V. Zozulya, O. Ryazanova et al, Biophys. Chem. 185(1), 39 (2014).
[5] V. Zozulya, O. Ryazanova et al, J. Fluoresc. 20(3), 695 (2010).
Acknowledgements: The author acknowledges the National Academy of Sciences of Ukraine for the financial support (Grant No. 0123U100628) and Wolfgang Pauli Institute (Vienna, Austria) for Pauli Postdoc research training scholarships “Data analysis in molecular biophysics” 2021/22.
